Pharmaceutical Regulatory Affairs

Biography

Biography: Tesfaye Zerihun

Abstract

Background & Objectives: Several plant products have been tested and found to possess antileishmanial activity. The present study was undertaken to evaluate antileishmanial activity of methanolic extract of Aloe otallensis, which is endemic plant to Ethiopia, on the promastigot stage of Leishmania aethiopica and Leishmania donovani comparing to standard drugs and also tried to screen its phytochemical constitute. Methods: Phytochemical screening was done on Methanolic extract of the exudates to the leaf of Aloe otallensis. Theserial dilution of the Extract was also evaluated for in vitro antileishmanial activity against Leishmania aethiopica and Leishmania Donavan on the strain of L. aethiopica (LDC/134) and L. Donovani (AM 563), which is found from the black lion hospital parasitology unit and the result was compared to standard drug of Sodium stibogluconate, milfostin and paramomycin. Result. The extract has an antileishmaniacidal activity with an IC50 of 141 μg/ml on L. ethiopica (LDC/134) and 123μg/ml on L. donovani (AM 563). The experimental data shows that relatively it has better activity than paramomycin and milfostin but less activity than sodium stibogluconate, which is given in Ethiopia as a first line drug. The data analyses was done by pad graph prison version 5 software after it was read by ELISA redder at the wave length of 650 nm. The phytochemical screening of the exudates of Aloe otallensis showed the presence of phenol, alkaloid and saponin. Conclusion: The methanolic extract of exudate of Aloe otallensis has a good anti leishmanisis activity relatively to paramomycin and milfostin and this activity may be attributed to phenol, Alkaloid and Saponin present in the plant. But it needs further analysis for the conformation of which constituent present in much concentration and to know which one have highest role.